|
KMID : 0355220070320030251
|
|
Journal of Korean Academy Oral Medicine
2007 Volume.32 No. 3 p.251 ~ p.261
|
|
|
Synthetic Chenodeoxycholic Acid Derivative HS-1200-Induced Apoptosis of Human Oral Squamous Carcinoma Cells
|
|
Kim In-Ryoung
Sohn Hyeon-Jin
Kwak Hyun-Ho
Kim Gyoo-Cheon
Park Bong-Soo
Choi Won-Chul
Ko Myung-Yun
An Yong-U
|
|
|
Abstract
|
|
|
Bile acids and synthetic its derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. Previous studies have been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis inducing activity on various cancer cells in vitro. It wasn’t discovered those materials have apoptosis induced effects on YD9 human oral squamous carcinoma cells. The present study was done to examine the synthetic bile acid derivatives(HS-1199, HS-1200) induced apoptosis on YD9 cells and such these apoptosis events. We administered them in culture to YD9 cells. Tested YD9 cells showed several lines of apoptotic manifestation such as activation of caspase-3, degradation of DFF, production of poly (ADP-ribose) polymerase(PARP) cleavage(HS-1200 only), DNA degradation(HS-1200 only), nuclear condensation, inhibition of proteasome activity, reduction of mitochondrial membrane potential(HS-1200 only) and the release of cytochrome c and AIF to cytosol. Between two synthetic CDCA derivatives, HS-1200 showed stronger apoptosis-inducing effect than HS-1199. Therefore HS-1200 was demonstrated to have the most efficient antitumor effect.
Taken collectively, we demonstrated that a synthetic CDCA derivative HS-1200 induced caspases-dependent apoptosis via mitochondrial pathway in human oral sqauamous carcinoma cells in vitro. Our data therefore provide the possibility that HS-1200 could be considered as a novel therapeutic strategy for human orall squamous carcinoma from its poweful apoptosis-inducing activity.
|
|
|
KEYWORD
|
|
|
Apoptosis, Synthetic CDCA derivatives, HS-1200, Human oral squamous cell carcinoma
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
|